Cyp27b1 ELISA Kit for Rat(Rattus norvegicus) | EIAab.comCyp27b1 ELISA Kit for Rat(Rattus norvegicus)

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Cyp27b1 (Gene name),

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (Protein name ), CP27B_RAT from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

Cyp27b1(Cyp27b);

Protein name:

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;

Alternative:

25-hydroxyvitamin D(3) 1-alpha-hydroxylase(VD3 1A hydroxylase);25-OHD-1 alpha-hydroxylase;Cytochrome P450 subfamily XXVIIB polypeptide 1;Calcidiol 1-monooxygenase;Cytochrome P450VD1-alpha;Cytochrome P450C1 alpha;Cytochrome p450 27B1;

Organism:

Rat (Rattus norvegicus).

General Annotation

Sub Unit:

N/A

Function:

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Subcellular Location:

Mitochondrion membrane

Protein Attributes:

Sequence length:

501

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

501:

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

KEGG:

rno:114700

Pfam:

PF00067

UniGene:

Rn.10847

Uniprot:

O35132

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ELISA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324h

Price:

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Manual:

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Packing:

96T

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ELISA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324r

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96T

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ELISA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324m

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MSDS:

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Packing:

96T

CLIA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324m

Price:

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MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

P10324Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Monoclonal Antibody for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Protein for Rat 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of CP21B into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	89-103
Plasma	93	86-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CP21B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	85-101%	83-103%	86-97%
EDTA plasma(n=5)	89-98%	87-99%	89-96%	90-95%
heparin plasma(n=5)	85-100%	86-101%	84-102%	88-108%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"The 25-hydroxyvitamin D 1-alpha-hydroxylase gene maps to the pseudovitamin D-deficiency rickets (PDDR) disease locus."
St Arnaud R. , Messerlian S. , Moir J.M. , Omdahl J.L. , Glorieux F.H.
J. Bone Miner. Res.12:1552-1559(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: Sprague-Dawley.
2.

"Cloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA."
Shinki T. , Shimada H. , Wakino S. , Anazawa H. , Hayashi M. , Saruta T. , Deluca H.F. , Suda T.
Proc. Natl. Acad. Sci. U.S.A.94:12920-12925(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Kidney.

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